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In our lab, we’re not just observing the AI revolution—we’re living it. We’re actively integrating AI, especially Large Language Models (LLMs), to fundamentally change how we do science. This isn’t about replacing your expertise; it’s about giving you superpowers to be more efficient, insightful, and impactful in your research, all while upholding the highest standards
All photos are 3D pictures, it was fun to try out the technology. Regrettably, the MRC defunded the MRC Cancer Unit and the School of Clinical Medicine could no longer support our Department. In the current academic job market, I am experiencing some uncertainties about where I will relocate. This is thus one of my
In early 2016, I was asked if I wished to speak at the discussion meeting “Conflict and Competition in Cellular Populations” in Bangalore, India organized by Dr Sandeep Krishna and Dr Sunil Laxman (NCBS). The title sounded so intriguing that I accepted without even checking the actual topic of the meeting. Then an adventure begun,
Why should you know FRET? Well, FRET is used when you do a real-time qPCR, or you might be using it in assays like HTRF, or to detect biochemical reactions in single living cells. You might measure protein-protein interactions, probe cell signalling, cell metabolism or nano-meter scale conformational changes. Or what about dimerization, protein –
In fluorescence microscopy, colocalization is the spatial correlation between two different fluorescent labels. Often, we tag two proteins in a cell with distinct fluorescent labels, and we look if and where the staining localizes. When there is a “significant overlap” between the two signals we say that the two molecules “colocalize” and we might use
Is it a cat? Is it a dog? Is the average between a cat and a dog a real thing, perhaps a caog or a doat? Not all science should be based on single cell detection, and there are plenty of cases where single cell measurements are superfluous. However, too often we fail to appreciate the huge mistakes
Industry, academia and healthcare often rely on fluorescence microscopy to see the fine architecture of materials, including biological ones. Fluorescence microscopy is particularly suited for biomedical studies because it can be gentle with biological materials permitting investigators to study biology in a non-destructive manner. Chemistry and genetic engineering then provide useful strategies to make samples
What has been the impact of fluorescence lifetime imaging microscopy to science and to the biomedical community in particular? Is FLIM a niche technique, one of those techniques that always promise but never deliver? The top 10 most cited papers Excluding reviews, the list of the top 10 most cited papers, albeit representing a very
Since a few months, the manuscript entitled “Multiplexed biochemical imaging reveals caspase activation patterns underlying single cell fate“, and authored by Maximilian W Fries, Kalina T Haas, Suzan Ber, John Saganty, Emma K Richardson, Ashok R Venkitaraman, Alessandro Esposito, is available as pre-print at the bioRxiv repository. It has started its journey through the peer-review process,
Well, I remember when I started this business, a beam stop was done with a recycled block of lead and reflections stopped with carton boxes 😉 Brown boxes, black carton catches fires, of course (tell this to my undergrad-self). Not any longer, of course! About ten years ago, I started the procurement and development of
Are you interested in cell biochemistry, but in single living cells, organoids or tissues? Is there a Western blot or IP you wished to do on a living sample? Or did you wish to see where in a cell a protein-protein interaction occurs. Well, if you are interested in quantifying a ligand concentration, a post-translational
NyxSense&NyxBits paper here. I am not fond of new achronyms or ‘cool’ names, but then… guilty! you got me, I am contributing to the proliferation of four letters acronyms and fancy names like others! Lately, I have introduced a new one, HDIM as for Hyper-Dimensional Imaging Microscopy. But that is another story, and in a
Project outcome published in Biophysical Journal in 2010. Esposito A*, Choimet JB, Skepper JN, Mauritz JMA, Lew VL, Kaminski CF, Tiffert T, “Quantitative imaging of human red blood cells infected with Plasmodium falciparum“, Biophys. J., 99(3):953-960 Most papers have an untold backstory that we cannot reveal in it so to focus on a main message and
Project outcome published in PLoS ONE in 2013. Esposito A*, Popleteeva M, Venkitaraman AR, “Maximizing the biochemical resolving power in fluorescence microscopy”, PLOS ONE, 8(10):e77392 After my 2007 theoretical work on photon-economy and acquisition throughput, I occasionally worked on a more general framework attempting to falsify my hypothesis that multi-channel or multi-parametric imaging techniques can