Education and post held

 

2012- Senior Investigator Scientist

                     MRC Cancer Unit at the University of Cambridge (UK)

 

2009-2011 EPSRC Postdoctoral Fellow (PI in EP/F044011/1 and /2)

University of Cambridge in 2009 and at then at the MRC Cancer Unit

 

2007-2008    Postdoctoral Research Associate

                     Department of Chemical Engineering and Biotechnology

                     Department of Physiology, Development and Neuroscience

                     University of Cambridge (UK)

 

2003-2006    PhD in Physics

                     Molecular Biophysics Group, Department of Physics – Debye Institute, Utrecht University (NL) 

                     Cell Biophysics Group, European Neuroscience Institute – Göttingen (DL)

 

1994-2002    Physics Graduation

                     Department of Physics, University of Genoa (IT)

                     National Institute for the Physics of Matter and the Italian National Research Council, Genoa (IT)

 

Awards and Research Grants

 

·          2013, Mid-Career Equipment Grant, Microscopy setup for Optogenetics (£200,0000)

·          2012, MRC Centennial Award (Project Award), "Inducible acute oncogene activation in the living cell (optogenetics)", £7k

·          2012, MRC Centennial Award (Training Award), ~2k to attend the Biophysical Society Meeting in Philadelphia

·          2009, Simon S, Bertoncini C and Esposito A, Innovation Competition Award "Super-resolution fluorescence imaging of biological polymers relevant to disease" (£5,000) by CambridgeSens

·          2008, EPSRC fellowship (EP/F044011/1) “Multiplexed measurement of molecular interactions using hyper-spectral imaging and multi-parametric detection” (£409,836)

·          2006, “Sergio Ciani” Award for the “best doctoral dissertation in biophysics” assigned by the Italian Society of Pure and Applied Biophysics (SIBPA)

 

Teaching and line management experience

 

Line management

·          From October 2013, managing a team of 2 PhD students and 2 post-doctoral scientists

·          From 2011, managed a number post-doctoral scientists and research assistants

·          From 2011, managing one staff member providing imaging Facilities service to the MRC Cancer Unit

·          Attended courses for line management and leadership provided by the MRC and Cambridge University

 

Teaching

·          Co-supervision of PhD and master students at Cambridge University and MRC Cancer Unit

·          Co-supervision of master students at the Neurosciences Program, International Max Planck Research School (IMPRS), Georg-August University – Göttingen

·          Lecturer and practical courses at workshops

·          Teaching at the private school “Istituto Europeo - Genova”: informatics (years 2000 and 2001)

 

 

 

Examiner

·          August 2013, External examiner, PhD Viva, University of Edinburgh (UK)

·          June 2013, External examiner, PhD Viva, King’s College London (UK)

 

Departemantal and university service

 

·          from 2014, Chair of the IT committee at the Hutchison/MRC Research Centre

o    assisted major infrastructural upgrades

·          from 2013, Member of the Local Trade Union Side at the MRC Cancer Unit

o    assisted the transition of the Unit from MRC to University of Cambridge

·          from 2011, direction of the Imaging Facilities at the Hutchison/MRC Research Centre

o    provided service to the institute by line managing the imaging facility manager and directing the development and procurement of new systems

o    directly involved in the procurement of high-end equipment with capital bids and tenders totalling several millions

o    shared responsibility for major infrastructural refurbishment

·          from 2009, Laser Safety Officer at the Hutchison/MRC Research Centre

o    established Laser Safety policies and risk assessments and contributed to the overall H&S decisional process at the Research Centre

 

Service to profession

 

Committee affiliations

From 2013, Light Microscopy Section Committee at the Royal Microscopical Society

 

Refereeing for international journals

Nature Methods, Biophysical Journal, Optics Express, Current Biology, Neurobiology of Disease, Journal of Microscopy, Journal of Biomedical Optics, Microscopy Research and Technique and others

 

Refereeing for funding agencies

Biotechnology and Biological Sciences Research Council UK, Engineering and Physical Sciences Research Council UK, Medical Research Council UK, Dutch Technology Foundation, Wellcome Trust UK

 

Professional affiliations

 

·          Robinson College, Cambridge (UK)

·          Scientific societies: Biophysical Society (from 2005) and Royal Microscopical Society (from 2007)

Organizational Experience

 

·          2015, Co-chair of the “FRET, fluorophores and novel techniques for biochemical imaging” session at the MMC2015 (Manchester, United Kingdom)

·          2011, Chair of the "Live Cell Microscopy II" session at Focus on Microscopy 2011 (Konstanz, Germany)

·          2008, Co-organizer of the “Theodor Förster international lecture series on quantitative fluorescence microscopy”

Department of Chemical Engineering, Gurdon Institute and Cancer Research UK, University of Cambridge

·          2007-2015, Initiator and developer of the educational outreach on microscopy “WikiScope” (www.wikiscope.org)

 

Technical expertise

 

Quantitative imaging (e.g., FLIM, hyperspectral, FAIM, HDIM, super-resolution), cancer biology, tumour imaging, molecular biology (FRET sensors design and cloning in general), optogenetics (e.g., photo-inducible oncogenes and CRISPER/CAS9 system), theory (e.g., Fisher information and photophysics), technology (sensor development and system integration), software development (data acquisition and analysis).

 

Full List of publications

 

Bibliometrics

·          Author of 41 publications, 15 of which as correspondent author (*), 17 as first or last author, including 4 book chapters.

·          h-index: 18 (Thomson Reuters) with ~710 citations (ResearcherID: A-8536-2008)

 

Articles

·          Popleteeva A, Stoppa D, Pancheri L, Gasparini L, Kaminski CF, Cassidy LD, Venkitaraman AR, Esposito A*, “An integrated array of smart pixels facilitates multidimensional biochemical and medical imaging”, in revision

·          Liang H, Esposito A, De S, Ber S, Collin P, Surana U, Venkitaraman AR, "Homeostatic control of the G2 checkpoint via polo-like kinase 1 engenders non-genetic heterogeneity in its fidelity and timing", Nat Comms.5, 4048

·          Esposito A* , Popleteeva M, Venkitaraman AR, “Maximizing the biochemical resolving power in fluorescence microscopy”, PLOS ONE, 8(10):e77392

·          Kiwanuka S, Laurila T, Jonathan F, Esposito A, Blomberg von der Geest K, Pancheri L, Stoppa D, Kaminski C, "Development of broadband cavity ring-down spectroscopy for biomedical diagnostics of liquid analytes", Analytical Chemistry, 84 (13):5489–5493

·          Esposito A* , "Beyond range: innovating fluorescence microscopy", Remote Sens. 4(1):111-119

·          Fereidouni F, Esposito A, Blab GA, Gerritsen HC, "A modified phasor approach for analyzing time-gated fluorescence lifetime images", J. Microsc. 244(3):248-258

·          Esposito A* , Bader AN, Schlachter SC, van den Heuvel DJ, Kaminski Schierle GS, Venkitaraman AR, Kaminski CF, Gerritsen HC, "Design and application of a confocal microscope for spectrally resolved anisotropy imaging", Opt. Express, 19(3):2546–2555

·          Kaminski Schierle GS, Bertoncini CW, Chan FTS, van der Goot AT, Schwedler S, Skepper J, Schlachter S, van Ham T, Esposito A, Kumita JR, Nollen EAA, Dobson CM, Kaminski CF, "A FRET sensor for non-invasive imaging of amyloid formation in vivo", ChemPhysChem, 12(3):673-680

·          Mauritz JMA, Seear R, Esposito A, Kaminski CF, Skepper J, Warley A, Lew VL, Tiffert T, "X-ray Microanalysis Investigation of the Stage-Related Changes in Na, K and Hemoglobin Concentration in Plasmodium Falciparum -Infected Red Blood Cells", Biophys. J., 100(6):1438-1445

·          Jeyasekharan AD, Ayoub N, Mahen R, Ries J, Esposito A, Rajendra E, Hattori H, Kulkarni RP, Venkitaraman AR, "DNA damage regulates the mobility of Brca2 within the nucleoplasm of living cells", Proc. Natl. Acad. Sci. USA, 107(50): 21937–21942.

·          Swietach P, Tiffert T, Mauritz JM, Seear R, Esposito A, Kaminski C, Lew VL, Vaughan-Jones RD, "Hydrogen ion dynamics in human red blood cells", J Physiol., 588(24):4995-5014

·          Mauritz J, Esposito A, Tiffert T, Skepper JN, Warley A, Yoon YZ, Cicuta P, Lew VL, Guck JR, Kaminski CF, "Biophotonic techniques for the study of malaria-infected red blood cells", Med Biol Eng Comput., 48(10):1055-63

·          Mauritz J, Tiffert T, Seear R, Lautenschlaeger F, Esposito A, Lew V, Guck J, Kaminski CF, "Detection of Plasmodium falciparum -infected red blood cells by optical stretching", J. Biomed. Opt., 15(3):030517

·          Esposito A* , Choimet JB, Skepper JN, Mauritz JMA, Lew VL, Kaminski CF, Tiffert T, "Quantitative imaging of human red blood cells infected with Plasmodium falciparum", Biophys. J., 99(3):953-960

·          Cheung W, Gill M, Esposito A, Kaminski CF, Courousse N, Chwetzoff S, Trugnan G, Keshavan N, Lever A and Desselberger U , "Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication", J. Virol. 84(13): 6782-6798

·          Schlachter S, Schwedler S, Esposito A, Moggridge GD, Kaminski GS and Kaminski CF , "A method to unmix multiple fluorophores in microscopy images with minimal a priori information", Opt. Express, 17(25):22747-22760

·          Cueff A, Dyrda A, Bouyer G, Egée S, Seers R, Esposito A, Tiffert T, Lew VL and Thomas SLY , "Effects of elevated intracellular calcium on the osmotic fragility of human red blood cells", Cell Calcium, 47(1):29-36

·          Wagner OI, Esposito A, Shen K, Wenzel D, Koehler B, Wouters FS and Klopfenstein DR , "Synaptic scaffolding protein SYD-2 clusters and activates kinesin-3 UNC-104 in C. elegans", Proc. Natl. Acad. Sci. USA, 106(46):19605-10

·          van Ham TJ, Esposito A, Kumita JR, Hsu SD, Kaminski-Schierle GS, Kaminski CF, Dobson CM, Nollen EAA  and Bertoncini CW , "Towards multiparametric fluorescent imaging of amyloid formation: Studies on a YFP model of alpha-synuclein aggregation", J Mol Biol 395(3):627-642

·          Mauritz J, Esposito A*, Ginsburg H, Kaminski CF, Tiffert T and Lew VL, "The Homeostasis of Plasmodium falciparum Infected Red Blood Cells", PLoS Comp Biol 5(4):e1000339.doi:10.1371/journal.pcbi.1000339

·          Schlachter S, Elder AD, Esposito A, Kaminski GS, Frank GH, van Geest LK, and Kaminski CF, "mhFLIM: Resolution of heterogeneous fluorescence decays in widefield lifetime microscopy", Opt Express 17(3):1557-1570

·          Elder AD, Domin A, Kaminski Schierle GS, Lindon C, Pine J, Esposito A, and Kaminski CF, "A quantitative protocol for dynamic measurements of protein interactions by FRET-sensitized fluorescence emission". Journal of the Royal Society Interface. J R Soc Interface 6(S1):S59-S81

·          Babuke T, Ruonala M, Meister M, Amaddii M, Genzler C, Esposito A, Tikkanen R, "Hetero-oligomerization of reggie-1/flotillin-2 and reggie-2/flotillin-1 is required for their endocytosis". Cell Signal, 21(8):1287-97

·          Papusheva E, Mello de Queiroz F, Dalous J, Han Y, Esposito A, Jares-Erijman E, Jovin TM, and Bunt G, "Dynamic conformational changes in the FERM domain of FAK are involved in focal adhesion behavior during cell spreading and motility". J Cell Sci 122, 656-666

·          Esposito A* , Tiffert T, Mauritz J, Schlachter S, Bannister LH, Kaminski CF, Lew VL (2008), "FRET Imaging of Haemoglobin Concentration in Plasmodium Falciparum Infected Erythrocytes", PLoS ONE, 3(11): e3780. doi:10.1371/journal.pone.0003780

·          Esposito A* , Gralle M, Dani MAC, Lange D, Wouters FS (2008), "pHlameleons: a family of FRET-based protein sensors for quantitative pH imaging", Biochemistry, Biochemistry, 47(49):13115–13126

·          Wouters FS and Esposito A (2008), "Quantitative analysis of fluorescence lifetime imaging made easy". HFSP Journal 2(1):7-11 (commentary paper)

·          Esposito A* , Gerritsen HC, Wouters FS (2007), “Optimizing frequency-domain fluorescence lifetime sensing for high throughput applications: photon-economy and acquisition speed”. J. Opt. Soc. Am. A 24(10):3261-3273

·          Esposito A* , Dohm CP, Bähr M and Wouters FS (2007), “Unsupervised Fluorescence Lifetime Imaging Microscopy for High-Content and High-Throughput Screening”. Mol. Cell. Proteomics 6:1446-1454

·          Esposito A* , Dohm CP, Kermer P, Bähr M, Wouters FS (2007), “α-synuclein and its disease-related mutants interact differentially with the microtubule protein tau and associate with the actin cytoskeleton”. Neurobiol. Dis. 26(3): 521-531

·          Dohm CP, Siedenberg S, Liman J, Esposito A, Wouters FS, Reed JC, Bähr M, and Kermer P (2006), “Bax inhibitor-1 protects neurons from oxygen-glucose deprivation”. J. Mol Neurosci. 29(1):1-8

·          Esposito A* , Gerritsen HC, Oggier T, Lustenberger F, Wouters FS (2006), “Innovating lifetime microscopy: a compact and simple tool for the life sciences, screening and diagnostics”. J. Biomed. Opt. 11(3):34016-34024

·          Esposito A* , Oggier T, Gerritsen HC, Lustenberger F, Wouters FS (2005), “All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing”. Opt. Express 13(24):9812-9821

·          Esposito A* , Gerritsen HC, Wouters FS (2005), “Fluorescence lifetime heterogeneity resolution in the frequency domain by lifetime moments analysis”.  Biophys. J. 89(6):1-14

·          Esposito A* , Cupello A, Pellistri F, Marchetti C, Robello M (2005), “Two-photon analysis of lead accumulation in cerebellar granule cells”. Neurochem. Res. 30(8):949-54

·          Cupello A, Esposito A, Marchetti C, Pellistri F, Robello M (2005), “Calcium accumulation in neurites and cell bodies of rat cerebellar granule cells in culture: effects on GABA(A) receptor function”. Amino Acids 28(2):177-182

·          Pellistri F, Cupello A, Esposito A, Marchetti C, Robello M (2004), “Two-photon imaging of calcium accumulation in rat cerebellar granule cells.”. Neuroreport 15(1):83-87

·          Esposito A , Federici F, Usai C, Cannone F, Chirico G, Collini M, Diaspro A (2004), “Notes On Theory And Experimental Conditions Behind Two-Photon Excitation Microscopy”. Microsc. Res. Tech.63(1):12-17

 

Book Chapters

·          Esposito A* , Schlachter S, Kaminski Schierle GS, Elder AD, Diaspro A, Wouters FS, Kaminski CF, Iliev AI, "Quantitative fluorescence microscopy techniques" in Cytoskeleton Methods and Protocols Ed.: Ray H. Gavin, Humana Press Series Methods in Molecular Biology). In press

·          Gerritsen HC, Agronskaia S, Bader A, Esposito A (2008), "Time Domain FLIM: theory and Instrumentation" in FRET & FLIM Imaging Techniques, Gadella TWG ed.; Series Laboratory techniques in biochemistry & molecular biology, van der Vliet PC and Pillai S eds.

·          Esposito A , Gerritsen HC, Wouters FS (2008), “Fluorescence lifetime imaging microscopy: quality assessment and standards”, in Standardization in Fluorometry: State-of-the Art and Future Challenges Ed.: Resch-Genger U (Springer Series Methods and Applications of Fluorescence, Ed.: Wolfbeis O)

·          Esposito A , Wouters FS (2004), “Fluorescence Lifetime Imaging Microscopy”, in Current Protocols in Cell Biology. Eds.:Bonifacino JS, Dasso M, Harford JB, Lippincott-Schwartz J, and Yamada KM, John Wiley & Sons, New York, NY.

 

Patent applications

·          2007, Esposito A, Wouters FS, Lustenberger F, Oggier T, “Apparatus and method for fluorescence lifetime imaging” EP1746410 and US 2007/0018116 A1 (bought by Carl Zeiss GmbH)

 

Thesis

·          PhD thesis: “Molecular and Cellular Quantitative Microscopy”, Cuvillier Verlag (Goettingen, Germany) ISBN-10: 3865378293; Prof. H.Gerritsen (supervisor) and Prof. F.Wouters  (co-supervisor)

·          Degree thesis: "Two-photon analysis of intracellular divalent ions in rat cerebellar granules culture” Prof. M.Robello (supervisor) and Dr. C.Marchetti (co-supervisor)

 

Oral communications

 

·          02.07.2015“FLIM and optogenetics: A systems microscopy approach”, MMC2015, Manchester (UK)

·          30.03.2015 “FLIM and optogenetics: A systems microscopy approach”, Focus on Microscopy, Goettingen (DL)

·          13.10.2014 (invited) “Beyond range: innovating fluorescence microscopy”, CMOS SPAD Applications Workshop, Edinburgh (UK)

·          04.04.2014 (invited) “Multiplexing biochemical reactions within the cell: new approaches and sensors”, Third Workshop on High-speed Imaging Sensors, Oxford (UK)

·          25.03.2013 “Hyperdimensional imaging and biochemical multiplexing”, Focus on Microscopy, Maastricht, (NL)

·          13.11.2012 (invited) "Imaging cell biochemistry: from state-of-the-art to Systems Microscopy", Biomolecular Interactions Analysis 2012 - Instruct Centre Training Course, Porto (PT)

·          14.09.2012 (invited) "Towards system microscopy: new tools to map cell biochemistry", Frontiers in Imaging: Ultrafast Non-linear Microscopy and Biophotonics User Group Meeting and Workshop, London (UK)

·          26.01.2012 (invited) "Nanobiotechnologies meet Systems Biology", ANIS3, Vipiteno (Italy)

·          19.04.2011 "Hyperdimensional imaging microscopy and tumour imaging", FoM2011, Konstanz (Germany)

·          28.01.2011 (invited), "Beyond range: innovating fluorescence microscopy", RISA2011, Trento (Italy)

·          12.09.2010 (invited), "Imaging biochemical pathways by hyper-dimensional imaging microscopy", SIBPA 2010, Arcidosso (Italy)

·          26.04.2010 (invited), "High-throughput high-content screening with biophysical imaging techniques: state-of-the-art and prospects", High Throughput Screening conference, London (UK)

·          30.03.2010, "Multiplexed measurement of molecular interactions by hyperdimensional imaging microscopy", FocusOnMicroscopy 2010, Shanghai, China

·          24.02.2010, "Multiplexed measurement of molecular interactions using hyper-spectral imaging and multi-parametric detection", Biophysical Society Meeting, San Franciso (USA)

·          14.10.2009 (invited), “Advanced optical tools for the characterization of Plasmodium facliparum infected red blood cells”, Advanced drug delivery and diagnostics, 13-16 October, Trieste (IT)

·          12.07.2009 (invited), "Quantitative imaging and the homeostasis of Plasmodium falciparum infected erythrocytes", European Biophysics Congress (EBSA2009), Genoa (IT)

·          14.04.2009, "Photon-statistics and Förster resonance energy transfer", Focus On Microscopy 2009, Krakow (PL)  

·          20.09.2008, "Quantitative imaging and the homeostasis of Plasmodium falciparum infected erythrocytes", SIBPA 2008, Rome (IT)

·          14.04.2008, "Fluorescence techniques for the investigation of the homeostasis of malaria infected red blood cells", Focus on Microscopy 2008, Osaka (JP) 

·          21.09.2006, “Fluorescence lifetime image based screening and quantitative FRET-based biosensors”, SIBPA2006, Palermo (IT)

·          10.04.2006, “Image based screening and unsupervised fluorescence lifetime imaging”, Focus on Microscopy 2006, Perth (AU)

·          10.10.2005, “Automated Fluorescence Lifetime Imaging Microscopy (FLIM) for (ultra)High-Throughput and High-Content Screening: theoretical and technological developments”, Dutch meeting on cellular and molecular biophysics, Lunteren (NL)

·          24.06.2003, “Two-photon analysis of lead accumulation in cerebellar granule cells”, INFM Meeting, Genoa (IT)

·          11.09.2002, “Two Photon Fluorescence Microscopy Study Of Calcium Accumulation In Cerebellar Granule Cells”, SIBPA2002, Trento (IT)

 

 

Other Lectures

 

·          03.02.2011, “Towards systems microscopy: new tools to map cell biochemistry”, Center for Applied Photonics, Konstanz (Germany), host: Prof. Elisa Ferrando-May

·          23.07.2010, “Optical sensors and current developments in the healthcare sector”, Lecture for Beijin University of Chemical Technology students, Dept. Chem. Eng and Biotech., Uni. Cambridge (UK), host: Dr. A. Fisher

·          18.06.2010, “Imaging biochemistry inside the cell”, Microscopy and Molecular Imaging in Cambridge Forum, Cambridge (UK), host: Dr. Sumeet Mahajan

·          30.10.2008, “Imaging biochemical pathways in the living cell”, Cluster of Excellence Macromolecular Complexes , Frankfurt (Germany), host: Prof. Jürgen Bereiter-Hahn

·          18.06.2008, “Imaging biochemical pathways in the living cell”, Fondazione Bruno Kessler, Trento (Italy), host: Dr David Stoppa

·          Advanced Microscopy Workshop ENI-Göttingen, lecturer and practical courses (years 2004 and 2005)

 

Posters presented at conferences

 

·          30.03.2015, “Fast and efficient fluorescence lifetime imaging microscopy”, Focus on Microscopy, Goettingen (DL)

·          25.03.2013, “The photon partitioning theorem: Enhancing biochemical resolution in microscopy”, Focus on Microscopy, Maastricht, (NL)

·          06.02.2013, “towards systems microscopy: achieving biochemical multiplexing by hyperdimensional imaging”, Biophysical Society Meeting, Philadelphia, USA

·          29.09.2009, “Biophysical microscopy and neurodegeneration”, Cambridge Clinical Neuroscience and Mental Health Symposium, Cambridge

·          19.03.2009, “FRET imaging and neurodegeneration”, CNS 2009: New Approaches in Neuroscience, Cambridge

 

languages

Italian (native) and English (fluent)

 

References

Available upon request